Experiment Description

Experiment Description

We previously showed that TLX is an enhancer of IFN-gamma induced STAT1-mediated transcription and immunity to Toxoplasma infection. Given the importance of this signaling pathway in macrophage differentiation and activation, we decided to test whether TLX might play a role in the biology of this critical immune cell type. In this experiment we retrovirally transduced Ckit+ fetal liver cells (purified over magnetic columns) with either control virus, or virus overexpressing TLX. Cells were then cultured for 5 days under multilineage conditions and cells lineage commitment was assessed by flow cytometry. We observed that ectopic expression of TLX in cKit+ progenitor cells skewed lineage commitment toward a myeolid fate. To determine the extent to which TLX modulated the phenotype of macrophages, we purified CD11b+ cells from cultures transduced with control virus (‘control’), cultures transduced with control virus and stimulated with IFN-gamma (‘IFNG’), and cultures transduced to express TLX (‘TLX’). Illumina microarrays were carried on all three populations isolated from three replicate experiments.