As the inaugural lecture for the course, we’ll spend this lecture discussing Illumina’s ‘Sequencing by Synthesis’ technology. We’ll also walk through the steps involved in planning for a transcriptomics experiment. After this lecture, jargon like flow cell, single-end, paired-end, indexing, cluster density, library, ribo-depletion, scRNAseq, and fastq will practically roll off your tongue.
Learning objectives
- Get familiar with jargon of RNAseq/HTS (paired-end, cluster density, fastq, index, etc)
- Understand Illumina’s “Sequencing by Synthesis” (SBS) technology
- Set priorities when planning a sequencing experiment
- Understand the basics of library prep for HTS
Reading
File formats produced by sequencing.
RNA-seqlopedia - Created by the Univ. of Orgeon, this is a great resource for understanding the entire RNAseq workflow.